MILK CLOTTING ACTIVITY OF RECOMBINANT CAMEL CHYMOSIN

One of the earliest biotechnological applications enzymes is cheese production. The high specific milk-clotting activity chymosin gives it an advantage over other proteases in industry. action based on hydrolysis bond between amino acid residues Phe105 and Met106 κ-casein molecule, which causes destabilisation casein micelles, coagulation milk its separation into a clot whey. A recombinant camel Camelus Bactrianus was obtained using yeast producer strain Pichia pastoris GS115/pGAPZαA/ProchymCB. purified by ion exchange chromatography. camelian against mare's studied as function pH temperature. It found that optimum value at maximum camel's with respect to observed 4.0 temperature 70°C. mare, cow goat 123, 9605 4650 units/mg, respectively. Recombinant shown have activity, sufficient produce curd cheese. yield from 6 l 936 grams. has prospects for use cow's goat's processing production technologies.